How to use HIV saliva test strips

We all know that due to the current openness of sexuality, AIDS is now spreading rapidly among the population. Nowadays, people may have AIDS, but they are not particularly sure. However, they are embarrassed to go to the hospital for examination. At this time, everyone will choose to buy dwarf saliva test strips at home and test themselves whether they have AIDS. But many people don’t know how to use this test paper, so now I will teach you how to use it.

HIV-1 gp41 and HIV-2 gp36 recombinant antigens were coated in the detection area of ​​the nitrocellulose membrane, and goat anti-human IgG F(ab')2 fragment polyclonal antibody was coated in the control area. During the test, wipe the upper and lower gum lines of the subject with an oral swab, then place the oral swab in a test tube containing about 1000 μl of sample buffer, mix evenly in the test tube, squeeze out the liquid in the oral swab, and discard the swab. Transfer approximately 200 μl of the sample mixture into a clean test tube for testing.

Insert the test strip vertically into the test tube containing the sample mixture. The light red protein A-colloidal gold marker on the test strip will dissolve, and the IgG in the sample (the reaction principle of other antibodies in the sample, such as IgA and IgM, is the same as that of IgG) will combine with the protein A/colloidal gold particles to form an "IgG-protein A-gold" complex. The "IgG-protein A-gold" complex will move upward along the test strip and first reach the detection area coated with HIV antigen.

If the sample contains HIV antibodies, the "IgG-Protein A-Gold" complex will bind to the antigen and remain on the test line, forming a light red line, which indicates a positive result. The depth of the line color is not proportional to the amount of antibodies in the sample. If there is no colored line in the test area, it means that the sample does not contain HIV antibodies, and the free "IgG-protein A-gold" complex continues to move to the top of the test paper and reaches the control area of ​​the test paper. The control area was coated with goat anti-human IgG F(ab')2 fragment and fixed on the control line of the test strip. The "IgG-Protein A-Gold" complex will bind to the goat anti-human IgG F(ab')2 fragment and be enriched on the control line, forming a light red line.

The appearance of the control line proves that the test strip is functioning normally. Regardless of whether the sample contains HIV-1/2 antibodies, in a valid test, a light red to purple control line should appear in the control area of ​​the test strip. The sample continues to move through the control zone and into the absorption zone. The function of the absorption zone is to adsorb the remaining "IgG-protein A-gold" complex, make it move within the test strip, and eliminate the background color.

The result can be read 20 minutes after the test strip is inserted into the diluted sample mixture, but no later than 45 minutes.

I think the usage of the HIV saliva test paper that I introduced today is already very clear. I think everyone should know how to use it with such simple words. But I have to remind you here that you should pay attention to body cleanliness in your daily sexual life, otherwise AIDS is a very difficult disease.