What does it mean that mycoplasma and chlamydia are negative? How to confirm the diagnosis?

What does it mean that mycoplasma and chlamydia are negative? How to confirm the diagnosis?

Chlamydia bacteria are widely present in our daily life, among which mycoplasma and chlamydia are the most common forms of pathogens. Mycoplasma and chlamydia are extremely easy to infect the human body. Regular testing of chlamydia bacteria is very necessary, but chlamydia and mycoplasma bacteria are not obvious in the early stages and are easily missed. Let’s take a look at what mycoplasma and chlamydia negative means and how to confirm the diagnosis in Xiamen.

Mycoplasma and Chlamydia are widely present in nature. It is not surprising to find Mycoplasma and Chlamydia in the human body. When the human immunity is reduced, they may multiply and cause disease.

Pathogenic mechanism of Chlamydia: It can inhibit the metabolism of infected cells, dissolve and destroy cells and lead to the release of lytic enzymes, the cytotoxic effect of metabolites, and cause allergic reactions and autoimmunity.

Pathogenic mechanism of mycoplasma: It does not invade the body's tissues and blood, but adheres to and settles in the epithelial cells of the respiratory tract or urogenital tract, causing cell damage through different mechanisms, such as acquiring lipids and cholesterol on the cell membrane to cause membrane damage, releasing neurotoxins, phosphatases, and hydrogen peroxide, etc.

1 Rapid antigen detection: Monoclonal antibody direct immunofluorescence method is often used to detect chlamydia in specimens. 2I-IsA method can also be used to add anti-chlamydia antibody enzyme-labeled antibody 18G and substrate for colorimetric quantitative detection. These two methods are simple and sensitive.

2 Routine blood test: Peripheral blood leukocyte count is generally normal, eosinophilia is increased.

3 PCR technology The conventional PCR technology has the advantages of rapidity, simplicity and specificity in detecting the specific DNA of Chlamydia pneumoniae. Its sensitivity is higher than that of cell separation technology, but it is not ideal in detecting throat swab specimens. The use of nested PCR (nPCR) detection can significantly improve its sensitivity.

4 X-ray examination: Chlamydia pneumonia chest radiographs are nonspecific and mostly show unilateral lower lobe infiltration, which manifests as segmental pneumonia. Severe cases show extensive bilateral pneumonia. Chlamydia trachomatis pneumonia chest radiographs show bilateral extensive interstitial and alveolar infiltration. Overinflation signs are common and lobar consolidation is occasionally seen.

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